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This paper presents a novel laser-induced fluorescence ( LIF) Lidar system for marine oil spilling detection. A bifurcated Y-type optical fiber and an optical collimating lens compose a coaxial configuration transceiver for this LIF-Lidar system. This LIF-Lidar system was further applied to measure the excitation spectra from floating oil slicks with different thicknesses on top of seawater at different distances. The system presents several advantages such as compact structure, stable optical path, and convenient operation, which offers a wide application prospect in ocean exploration.