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目的探讨小柴胡汤对病毒性心肌炎动物模型白细胞介素2(IL-2)、肿瘤坏死因子α(TNFα)和T淋巴细胞亚群的作用。方法将40只6~8周龄、体质量15~19 g雌性BALB/c小鼠随机分成治疗组和对照组,每组20只。经腹腔接种亲心肌细胞株柯萨奇病毒B3建立心肌炎模型,用小柴胡汤灌胃给药治疗。分别于治疗后5、7、15和21 d检测血清中的IL-2、INFα和血液中的T淋巴细胞亚群。结果病毒感染后5 d,治疗组小鼠IL-2活性无明显增高,与对照组比较差异无统计学意义;感染后7~21 d,治疗组小鼠IL-2活性逐渐增高,与对照组比较差异有统计学意义[7d:(83.4±4.6)mg/Lvs(72.7±4.2)mg/L,P<0.05;15d:(86.7±5.1)mg/Lvs(69.4±4.5)mg/L;21d:(95.3±4.8)mg/Lvs(64.2±4.6)mg/L]。病毒感染后5~21 d,治疗组小鼠血清中TNFα水平逐渐增高,与对照组比较差异有统计学意义[5d:(158.8±11.3)mg/Lvs(87.2±13.7)mg/L,P<0.01;7d:(173.7±12.5)mg/Lvs(109.6±12.4)mg/L,P<0.01;15d:(294.2±16.2)mg/Lvs(127.5±15.1)mg/L,P<0.01;21d:(366.7±18.6)mg/Lvs(145.3±18.3)mg/L,P<0.01]。病毒感染后5 d,治疗组小鼠外周血中CD4+和CD8+均无明显增高,与对照组比较差异无统计学意义;感染后7~21 d,治疗组小鼠外周血中CD4+和CD8+均逐渐增高,与对照组比较差异有统计学意义。结论小柴胡汤具有调节免疫应答功能,从而对病毒性心肌炎起到有效治疗作用。
Objective To investigate the effects of Xiao Chai Hu Tang on interleukin 2 (IL-2), tumor necrosis factor-α (TNFα) and T-lymphocyte subsets in animal models of viral myocarditis. Methods Forty female BALB / c mice aged 6-8 weeks and weighing 15-19 g were randomly divided into treatment group (n = 20) and control group (n = 20). After inoculation of cardiomyocytes Coxsackievirus B3 in myocardium, the model of myocarditis was established and treated with Xiao Chai Hu Tang by intragastric administration. Serum IL-2, INFα and T lymphocyte subsets in blood were detected at 5, 7, 15 and 21 d after treatment respectively. Results At 5 days after infection, the activity of IL-2 in the treated group was not significantly higher than that in the control group. At 7 and 21 days after infection, the activity of IL-2 in the treated group increased gradually. Compared with the control group The difference was statistically significant (7d: (83.4 ± 4.6) mg / L vs (72.7 ± 4.2) mg / L, P <0.05; 15d: (86.7 ± 5.1) mg / L vs 69.4 ± 4.5 mg / L : (95.3 ± 4.8) mg / L vs (64.2 ± 4.6) mg / L]. The levels of TNFα in serum of the treated mice gradually increased from 5 to 21 days after virus infection, which was statistically significant compared with the control group [5d: (158.8 ± 11.3) mg / L vs 87.2 ± 13.7 mg / L, P < 0.01; 7d: 173.7 ± 12.5 mg / L vs 109.6 ± 12.4 mg / L, P <0.01; 15d: (294.2 ± 16.2) mg / L vs 127.5 ± 15.1 mg / (366.7 ± 18.6) mg / L vs (145.3 ± 18.3) mg / L, P <0.01]. At 5 days after infection, CD4 + and CD8 + in the peripheral blood of the mice in the treatment group were not significantly increased compared with the control group, and there was no significant difference between the two groups Increased, compared with the control group, the difference was statistically significant. Conclusion Xiao Chai Hu Tang has the function of regulating immune response, and thus play an effective therapeutic effect on viral myocarditis.