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目的建立用HPLC法测定氨咖敏片中马来酸氯苯那敏含量的方法。方法用KromasilODS-C18色谱柱(250×4.6mm,5μm),以乙腈-0.3%十二烷基硫酸钠溶液-磷酸(60:40:0.02)(用三乙胺调PH值至3.3)为流动相,流速为1.0m·lmin-1,检测波长为215nm。结果马来酸氯苯那敏在10.03～30.09μg·ml-1范围内呈线性关系,r=0.9997,平均回收率为99.86%,RSD=0.3%(n=5)。结论本法适用于氨咖敏片中马来酸氯苯那敏的含量测定。
OBJECTIVE To establish a HPLC method for the determination of chlorphenamine maleate in ammonia-caffeine tablets. Methods The mobile phase was eluted with Kromasil ODS-C18 column (250 × 4.6 mm, 5 μm) using acetonitrile-0.3% sodium dodecyl sulfate-phosphoric acid (60:40:.02) Phase, the flow rate of 1.0m · lmin-1, detection wavelength of 215nm. Results Chlorphenamine maleate showed a linear relationship in the range of 10.03 ~ 30.09 μg · ml-1, r = 0.9997. The average recovery was 99.86% with RSD = 0.3% (n = 5). Conclusion This method is suitable for the determination of chlorphenamine maleate in ammonia-caffeine tablets.