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目的:研究慢性重型乙型肝炎患者外周血树突状细胞(DC)表型、免疫功能和分泌细胞因子的特性。方法:从20例慢性重型乙型肝炎患者和10例健康人外周血中分离单个核细胞培养DC,用流式细胞仪检测DC的表面标志CD80和CD86的表达。用3H-TdR掺入法检测DC诱导混合性淋巴细胞反应(MLR)的能力。并用ELISA方法检测MLR中细胞因子IL-12的分泌水平。结果:慢性重型乙型肝炎患者组的DC细胞CD80、CD86的表达率较正常人组高(P<0.05),诱导MLR的能力也较强(P<0.05),MLR中细胞因子IL-12的分泌水平也较高(P<0.05)。结论:慢性重型乙型肝炎患者外周血树突状细胞免疫功能亢进,免疫刺激能力也较高。
Objective: To study the characteristics of dendritic cell (DC) phenotype, immune function and cytokine secretion in peripheral blood of patients with chronic severe hepatitis. Methods: Mononuclear cells (DCs) were isolated from peripheral blood of 20 patients with chronic severe hepatitis B and 10 healthy controls. The expression of CD80 and CD86 on the surface of DC were detected by flow cytometry. The ability of DC to induce mixed lymphocyte reaction (MLR) was examined by 3H-TdR incorporation. The secretion of cytokine IL-12 in MLR was detected by ELISA. Results: The expression of CD80 and CD86 in DC patients with chronic severe hepatitis B was significantly higher than that in normal controls (P <0.05), and the ability of inducing MLR was also stronger (P <0.05). The levels of cytokines IL-12 Secretion was also higher (P <0.05). Conclusion: The peripheral blood dendritic cells in patients with chronic severe hepatitis B have hyperstimulation and immunostimulatory capacity.