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目的分析和评价德宏州结核病实验室室间质量控制状况,为确保结核病项目实施提供科学依据。方法采用双盲法考核各县市结核病痰检人员制片、染色、读片、结果判定等技术水平,并进行统计分析。结果8次抽查各县市疑似肺结核病痰涂片893张,阳性130张,阳性率14.56%;涂片的痰细胞合格率86.5%,涂抹大小符合率98%,痰膜厚薄符合率84.6%,染色符合率98%,脱落符合率100%,结果判定符合率100%,质量控制覆盖率100%,仅存在细菌量化的微小误差。结论德宏州各县市结核病痰涂片菌检技术符合国家结核病项目要求,对结核病项目病人的发现提供了技术保障。
Objective To analyze and evaluate the quality control of laboratory in Dehongzhou tuberculosis laboratory and provide a scientific basis for ensuring the implementation of tuberculosis project. Methods The double blind method was used to assess the technical level of production, staining, reading and determination of tuberculosis sputum examination staff in all counties and counties, and statistical analysis was made. Results Eighty-three samples of sputum smear were collected from 8 counties in each county. The positive rate was 14.56%. The pass rate of sputum smear was 86.5%, the coincidence rate of sputum smear was 98%, the coincidence rate of smear thickness was 84.6% Dyeing coincidence rate of 98%, off 100%, the results of the line with the rate of 100% compliance, quality control coverage of 100%, there is only a slight error of bacterial quantification. Conclusion The detection of sputum smear of tuberculosis in all counties and cities in Dehong meet the requirements of national tuberculosis project and provide technical support for the detection of tuberculosis patients.