论文部分内容阅读
目的:探讨明睛颗粒干预实验性脉络膜新生血管(CNV)的药效及作用机制。方法:建立实验性CNV模型,将青紫蓝兔随机分为空白对照组,模型组,明睛颗粒高、中、低剂量组,连续给药28d,通过眼底彩照、荧光素眼底血管造影术、组织切片对治疗效果进行评价;检测CD14和CD206阳性细胞在眼部的表达情况。结果:明睛颗粒3个剂量均能减少眼底荧光渗漏的面积(P<0.05),对眼底组织病变有保护作用,中剂量组效果最明显。免疫组化结果显示,模型组CD14阳性细胞明显增多(P<0.05),CD206阳性细胞较少;明睛颗粒组的CD14阳性细胞没有明显减少,但CD206阳性细胞显著性增多(P<0.05)。结论:明睛颗粒3个剂量对CNV的形成和生长均有一定抑制作用,中剂量效果最好。巨噬细胞参与了CNV形成,以Ⅰ型为主,明睛颗粒可诱导巨噬细胞向Ⅱ型转化。
Objective: To investigate the effect and mechanism of Mingjing granule on experimental choroidal neovascularization (CNV). Methods: The experimental model of CNV was established. The rabbits were randomly divided into blank control group, model group and Mingjing granule high, medium and low dose groups for continuous administration for 28 days. The fundus pictures, fundus fluorescein angiography, The therapeutic effect was evaluated by slicing. The expression of CD14 and CD206 positive cells in the eye was detected. Results: All the three doses of Mingjing Granule could reduce the area of fundus fluorescein leakage (P <0.05), and protect the fundus from the lesions of the fundus. The middle dose group was the most effective. The results of immunohistochemistry showed that the number of CD14 positive cells in the model group was significantly increased (P <0.05) and the number of CD206 positive cells was less in the model group. The CD14 positive cells in the Mingjing granule group were not significantly reduced, but the CD206 positive cells were significantly increased (P <0.05). Conclusion: The three doses of Mingjing Granule can inhibit the formation and growth of CNV, and the middle dose has the best effect. Macrophages are involved in the formation of CNV, mainly in type I, and Mingjing granules can induce the conversion of macrophages to type II.