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目的：总结人感染H7N9禽流感病例院内感染的细菌谱及耐药性特点，为其经验性使用抗菌药物提供参考依据。方法回顾性分析苏州地区重症人感染H7N9禽流感病例，住院期间采集各种病原学标本，所有标本均采用K-B法进行抗菌药物敏感试验，记录细菌培养及药敏试验结果，结果按CLSI 2010年版判断。结果入组的10例患者共培养出细菌79株，以革兰阴性杆菌为主，其中铜绿假单胞菌和洋葱伯克霍尔德菌出现率最高，均占27.85%，其次为鲍曼不动杆菌、粘质沙雷菌和肺炎克雷伯杆菌，分别占22.78%、11.39%和5.06%。鲍曼不动杆菌整体高度耐药，仅对头孢哌酮舒巴坦较为敏感。铜绿假单胞菌对哌拉西林他唑巴坦敏感性最高，对头孢他啶及亚胺培南耐药率高，洋葱伯克霍尔德菌整体耐药率较低，但对丁胺卡那耐药。粘质沙雷菌对青霉素及头孢类高度耐药。肺炎克雷伯杆菌对二、三代头孢菌素均有较高的敏感性，但对青霉素类耐药。结论当H7N9禽流感患者合并院内感染，但病原学尚不明确时，根据院内感染的细菌谱和耐药性特点选用抗菌药物尤为重要。“,”Objective To summarize the characteristics of bacterial spectrum and drug resistance of nosocomial infection in patients infected with avian influenza A H7N9 virus, in order to provide reference for the rational use of antimicrobial agents.Methods A retrospective analysis of Suzhou area of severe human infection bird flu cases hospitalized during the acquisition of various samples of bacterial culture and drug susceptibility test results, all specimens were treated with K-B antimicrobial susceptibility testing with a result according to CLSI 2010 judgment.Results All the 10 patients were cultured bacteria 79 strains, dominated by Gram negative bacilli, the occurrence of Pseudomonas aeruginosa bacteria and onion Burkholderia bacteria was the highest account for 27.85%, followed by Bauman Acinetobacter, Serratia marcescens and pneumonia Klebsiella, accounted for 22.78%,11.39% and 5.06% respectively. Bauman Acinetobacter was highly resistant to antibiotics, while sensitive to cefoperazone sulbactam. For Pseudomonas aeruginosa, the sensitivity to piperacillin tazobactam is the highest, while the resistant rate to ceftazidime and imipenem was high. Burkholderia cepacia has lower rates of Antimicrobial resistance, but resistant to amikacin. Serratia marcescens was highly resistant to penicillin and cephalosporin. Klebsiella pneumoniae had higher sensitivity to the second or third generation of the cephalosporin, but it was resistant to penicillin.Conclusion When the patients who is infected with avian influenza A H7N9 virus suffered from nosocomial infection, while the etiology is not clear, it is important to select antibiotics according to the bacterial spectrum and drug resistance.