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夏威夷铁树Cordyline terminalis(L)Kunth,是一种木本观赏植物,它的梢端可在试管内用经过一些改进的培养基进行繁殖,也可用铁树顶端分生组织邻近幼叶的愈伤组织进行分化繁殖.应用组织培养技术能加速铁树的繁殖,以满足当地的需要,并可得大量无病虫害的植株远销国外.这种方法可应用于草本植物也可应用于木本单子叶植物.本试验所采用的繁殖方法是经过改进的常规甘蔗组织培养法,从生长茁壮的铁树,取一段长2.5厘米的梢端,表面用95%酒精消毒,然后浸在95%酒精内1分钟,再浸1%的次氯酸钠20分钟,取出后用消毒蒸馏水冲洗三次,除去外面的组织,将梢端切成3毫米长的小块块,放入基本Ms(Murashiga与Skoog)培养基培养.在每升培养基内加3毫克的2.4—D;1毫克的硫胺素盐酸化合物;100毫克的肌醇;10%的椰汁(按体积计算);20克蔗(米广)和9克的琼脂.加入琼脂之前,把PH调至5.6,在28—30℃与1千勒(SylvaniaGRO—Lux F40—GRO)的持续光照下培养.培养2至4周,在梢端裸露的切口表面上长出愈伤组织.愈伤组织必需转移至不含2.4—D的基本培养基才能完成小苗的分化.在最后转移至分化培养基之前,每月将愈伤组织转移至基本培养基里2—3次可产生大量的小苗.含有2.4—D的培养基,不能诱导出根.
Cordyline terminalis (L) Kunth is a woody ornamental plant whose tips can be propagated in test tubes with some improved medium or with the callus of young leaves near the apical meristem of the tree top Differentiation and propagation.Using tissue culture technology can speed up the reproduction of the tree to meet the needs of the local and get a large number of pest-free plants are exported to foreign countries.This method can be applied to herbs can also be applied to woody monocots. The breeding method used in the experiment was an improved conventional sugarcane tissue culture method, from growing robust tree, taking a 2.5 cm long tip, disinfecting the surface with 95% alcohol, then immersing it in 95% alcohol for 1 minute and then dipping 1% sodium hypochlorite for 20 minutes, rinsed three times with sterile distilled water after removal, removed from the outer tissue, and the tip was cut into small pieces of 3 mm in length and plated on basic Ms (Murashiga and Skoog) medium. Base plus 3 mg of 2.4-D; 1 mg of thiamine hydrochloride compound; 100 mg of myo-inositol; 10% of coconut milk by volume; 20 g of cane (meter wide) and 9 g of agar. Prior to agar, adjust pH to 5.6 at 28-30 ° C 1 SlerGRO-Lux F40-GRO, and cultured for 2 to 4 weeks to grow callus on the bare surface of the shoot tip Callus must be transferred to a basal 2.4-D free Medium to complete the differentiation of the seedlings.Transfer the callus to the basal medium every month for 2-3 times to produce a large number of seedlings per month before the final transfer to the differentiation medium.The culture medium containing 2.4-D can not be induced root.