论文部分内容阅读
目的:建立复方桑忍外洗颗粒的质量标准。方法:采用薄层色谱法鉴别了复方桑忍外洗颗粒中的大黄、黄柏和忍冬藤,采用高效液相色谱法对复方桑忍外洗颗粒中的大黄酸和大黄酚进行含量测定,以甲醇-0.1%磷酸溶液(85∶15)为流动相;检测波长254 nm。结果:大黄、黄柏和忍冬藤的鉴别方法专属性强;大黄酸、大黄酚分别在1.36~6.80μg/m L、3.08~15.4μg/m L浓度范围内与各自峰面积呈良好的线性关系(r=1.000),平均加样回收率为101.3%、99.5%,RSD为1.21%、1.09%。结论:所建立的方法操作简单、专属性强、重现性好,可作为复方桑忍外洗颗粒的质量控制方法。
Objective: To establish the quality standard of compound mulberry and rinse granules. Methods: Rhubarb, Phellodendron chinense and Houttuynia cordata Thunb were removed by thin-layer chromatography (TLC). The content of rhein and chrysophanol in compound Wulin granules was determined by high performance liquid chromatography -0.1% phosphoric acid solution (85:15) as mobile phase; detection wavelength 254 nm. Results: The identification methods of Rhubarb, Phellodendron chinense and Honeysuckle were highly specific. Rhein and chrysophanol had good linear relationship with their peak areas in the range of 1.36-6.80μg / m L and 3.08-15.4μg / m L, respectively r = 1.000). The average recoveries were 101.3%, 99.5%, RSD 1.21%, 1.09%. Conclusion: The established method has the advantages of simple operation, strong specificity and good reproducibility. It can be used as a quality control method for the compound mulberry-tuber granules.