Objective:To investigate the antiproliferative and anti-metastasis effect of Xihuang Pill(西黄丸,XP) on human colorectal cancer cell and to explore the molecular mechanism by which it produces the effects.Methods:Highly metastatic human colorectal cancer cell line LoVo was treated with low-,medium-,and highdose XP-containing serum(XP-L,XP-M,XP-H) groups for 48 h,cells intervened with no drug rat serum and PD98059[extracellular signal-regulated kinase(ERK) inhibitor]as negative and positive controls(NC and PC)groups.Cell proliferation assay was made using cell counting kit-8(CCK8).The 8 μm pore-size transwell chamber and 4’,6-diamidino-2-phenylindole(DAPI) staining were applied to examine the ability of invasion and migration of the cells.The protein expression of ERK1/2,zinc finger E-box-binding homeobox 1(ZEB1),Scrib and lethal giant larvae homolog 2(Lgl2) was detected by Western blotting while the relative mRNA quantity of E-cadherin,N-cadherin,Occludin and junctional adhesion molecule-1(JAM1) was measured by realtime fluorescent quantitative polymerase chain reaction(RT-qPCR).Results:XP induced a dose-dependent suppression on the proliferation of LoVo cells(P<0.05 or P<0.01),with the inhibition rates varied from 27.30%to31.08%.Transwell assay showed that when preprocessed with PD98059 and XP-containing serum,the number of cells that passed the filter decreased significantly compared with that of NC group(P<0.05 or P<0.01).Moreover,XP inhibited the protein expression of ERK1/2 and ZEB1(P<0.05);and up-regulated the protein expression of Scrib and Lgl2(P<0.05).The mRNA levels of E-cadherin,Occludin and JAM1 of the XP intervened groups and PC group markedly ascended(P<0.05) while that of N-cadherin showed a descending tendency(P>0.05).Conclusion:XP intervention suppressed the ability of proliferation,invasion and migration of the LoVo cells.Regulating ZEB1-SCRIB Loop so as to recover epithelial phenotype and apical junctional complex might be one of the mechanisms by which XP produces the anti-metastasis effect. Objective: To investigate the antiproliferative and anti-metastasis effect of Xihuang Pill on human colorectal cancer cell and to explore the molecular mechanism by which it produces the effects. Methods: Highly metastatic human colorectal cancer cell line LoVo was treated with low-, medium-, and high-density XP-containing serum (XP-L, XP-M, XP-H) groups for 48 h, cells intervened with no drug rat serum and PD98059 [extracellular signal-regulated kinase inhibitor] as negative and positive controls (NC and PC) groups. Cell proliferation assay was made using cell counting kit-8 (CCK8). The 8 μm pore-size transwell chamber and 4 ’, 6-diamidino- ) staining were applied to examine the ability of invasion and migration of the cells. The protein expression of ERK1 / 2, zinc finger E-box-binding homeobox 1 (ZEB1), Scrib and lethal giant larvae homolog 2 (Lgl2) was detected by Western blotting while the relative mRNA quantity of E-cadherin, N-cadherin, Occludin and junctional adhesion mo Results: XP induced a dose-dependent suppression of the proliferation of LoVo cells (P <0.05 or P <0.01), with the inhibition inhibition rate of leukemia-1 (JAM1) was measured by realtime fluorescent quantitative polymerase chain reaction varied from 27.30% to 31.08%. Transwell assay showed that when preprocessed with PD98059 and XP-containing serum, the number of cells that passed the filter decreased significantly with that of NC group (P <0.05 or P <0.01) , XP inhibited the protein expression of ERK1 / 2 and ZEB1 (P <0.05); and up-regulated the protein expression of Scrib and Lgl2 (P <0.05). The mRNA levels of E-cadherin, Occludin and JAM1 of the XP intervened groups and PC group markedly ascended (P <0.05) while that of N-cadherin showed a descending tendency (P> 0.05) .Conclusion: XP intervention suppressed the ability of proliferation, invasion and migration of the LoVo cells.Regulating ZEB1-SCRIB Loop so as to recover epithelial phenotype and apical junctional complex might be one of the mechanisms s by which XP produces the anti-metastasis effect.