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目的 探讨自拟中药方———脑复聪颗粒对脑缺血小鼠记忆能力及大脑皮层超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH -Px)活性的影响。方法 采用双颈总动脉闭塞 -缺血 -再灌术造成学习记忆障碍小鼠模型。小鼠记忆能力以Morris圆形水迷宫的潜伏期作为指标。结果 小鼠脑缺血后 7d潜伏期〔(82 3± 3 2 0 )s〕比假手术组〔 (3 5 3± 3 7 9)s〕延长 (P <0 0 1) ;脑复聪组〔 (3 0 6± 3 1 3 )s〕恢复正常 (P <0 0 1)。脑缺血小鼠大脑皮层SOD和GSH -Px活性 (68 5 9± 2 5 5 ,10 88± 1 0 1)比假手术组 (79 68± 5 69,13 0 9± 2 5 3 )降低 ,脑复聪及当归芍药散治疗组提高到正常水平 (77 5 6± 4 88,13 3 5± 3 3 8;75 77± 6 88,13 3 2± 2 69) (P <0 0 5～ 0 0 1)。结论 脑复聪颗粒对脑缺血所致的智能障碍有保护作用 ,其机制可能与提高SOD和GSH -Px活性、对抗自由基损伤有关。
Objective To investigate the effect of Naofucong granule on the memory ability and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in cerebral cortex of mice. Methods A mouse model of learning and memory impairment caused by occlusion of both common carotid artery and ischemia - reperfusion was established. Mice memory ability to Morris circular water maze latency as an indicator. Results Compared with the sham operation group, the latency of 7 days (82 3 ± 3 2 0 s) in mice was longer than that in sham operation group (P <0.01) (3 0 6 ± 3 1 3) s〕 returned to normal (P 0 01). The activity of SOD and GSH-Px in the cerebral cortex of cerebral ischemic mice (68 59 ± 25 5, 10 88 ± 1 0 1) was lower than that of sham operation group (79 68 ± 5 69,13 0 9 ± 25 3) Naofucong and Danggui Shaoyao San in the treatment group increased to the normal level (77 56 ± 4 88,13 3 5 ± 3 3 8; 75 77 ± 6 88,13 3 2 ± 2 69) (P 0 05 0 0 1). Conclusion Naofucong granule has a protective effect on cerebral ischemia-induced mental retardation. The mechanism may be related to the increase of SOD and GSH-Px activity and the antagonism of free radical damage.