阴道毛滴虫染色体数目及染色体核型分析,国内尚未见报道。本文采用空气干燥法制备阴道毛滴虫染色体的标本,效果良好,现报告如下。从患者阴道后穹隆拭取分泌物,接种于肝浸汤培养基中(内含10％小牛血清,100u/ml青霉素,链霉素100μg/ml),37℃孵育48—72h。收获前1.5h加入秋水仙素(最终浓度 The chromosome number and karyotype analysis of Trichomonas vaginalis have not been reported in China. This article uses air drying method to prepare chromosome specimens of Trichomonas vaginalis. The results are good. The report is as follows. The secretions were swabbed from the patient’s posterior vaginal vault and inoculated into a liver soaking medium (containing 10% calf serum, 100u/ml penicillin, streptomycin 100 μg/ml) and incubated at 37°C for 48-72 hours. Add colchicine (final concentration) 1.5 h before harvest