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为了研究黄叶扁柏(Chamaecyparis obtusa var.breviramea f.crippsii)的化学成分,用硅胶柱、Sephadex LH-20、C18反相硅胶柱色谱、制备液相等色谱方法从其枝叶中分离得到6个化合物,通过波谱方法将它们的结构分别鉴定为(4S)-4-isopropylcyclohex-1-enecarboxylic acid 4-O-β-D-glucopyranoside(1)、(4R)-p-menth-1-ene-7,8-diol 7-O-β-D-glucopyranoside(2)、skimmin(3)、7-[[6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one(4)、stigmast-4-en-3-one(5)和1,4-benzenedicarboxylic acid 1-butyl-4-(2-methylpropyl)ester(6)。其中化合物1为新化合物,化合物2～6为首次从该植物中分离得到。体外细胞毒活性筛选发现,黄叶扁柏枝叶的甲醇提取物在人A549、BGC-823、Du145和MDA-MB-231肿瘤细胞株上均显示一定的细胞毒活性,其IC50值分别为0.94、1.07、0.95和0.96μg.mL 1,但化合物1、2和3在人HeLa、BGC-823和A549细胞株上均无明显细胞毒活性。
In order to study the chemical constituents of Chamaecyparis obtusa var. Breviramea f.crippsii, six compounds were isolated from the branches of the leaves using silica gel column, Sephadex LH-20, C18 reverse phase silica gel column chromatography and preparative liquid chromatography Their structures were identified by spectroscopic methods as (4S) -4-isopropylcyclohex-1-enecarboxylic acid 4-O-β-D-glucopyranoside (1), 8-diol 7-O-β-D-glucopyranoside (2), skimmin (3), 7 - [[6-O- (6-deoxy-α-L-mannopyranosyl) 2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1,4-benzenedicarboxylic acid 1-butyl-4- (2-methylpropyl) ester (6). Among them, compound 1 was a new compound, and compounds 2 ~ 6 were isolated from this plant for the first time. The results of in vitro cytotoxic activity assay showed that the methanol extracts from the branches and leaves of Cotyledonum cypress showed some cytotoxic activity on human A549, BGC-823, Du145 and MDA-MB-231 tumor cell lines with IC50 values of 0.94, 1.07 , 0.95 and 0.96μg.mL 1, respectively, but compounds 1, 2 and 3 showed no obvious cytotoxic activity on human HeLa, BGC-823 and A549 cell lines.