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目的 了解硫贲妥钠麻醉在中枢神经系统的作用部位 ,为进一步探讨全身麻醉作用机制打下基础。方法 用SD大鼠 2 4只 ,分别从静脉注射硫贲妥钠 15、2 0和 2 5mg·kg-1麻醉 1h ,应用c fos基因免疫组织化学法 ,光镜观察Fos蛋白阳性神经元在大脑的分布并计数。结果 研究表明硫贲妥钠麻醉在大脑有 15个Fos阳性神经核团参与麻醉调控作用 ,分别是梨状皮层、伏核、杏仁基底外侧核、外侧缰核、弧束核、外侧僵核、下丘脑室旁核、丘脑室旁核、膝状体腹下核、背外侧膝状核 ,视上核、视交叉上核、视前腹侧核、海马回嗅觉小岛和乳头状核。结论 硫贲妥钠麻醉在中枢神经系统具有明确的作用位点。
Objective To understand the role of Thiorbenzamide anesthesia in the central nervous system and lay the foundation for further exploration of the mechanism of general anesthesia. Methods 24 SD rats were anesthetized with intravenous injection of 15, 20 and 25 mg · kg-1 of thiopental respectively for 1 h. The expressions of c-fos gene immunohistochemistry and light microscopy were used to observe the expression of Fos protein positive neurons in brain The distribution and count. Results The study showed that there were 15 Fos-positive neurons in the brain involved in the anesthetic regulation of pilocarpine-induced anesthesia, namely, piriform cortex, nucleus accumbens, basolateral lateral alveolar nucleus, lateral habenular nucleus, Hypothalamic paraventricular nucleus, thalamic paraventricular nucleus, geniculate nucleus, dorsolateral geniculate nucleus, supraoptic nucleus, suprachiasmatic nucleus, preoptic ventral nucleus, hippocampus back olfaction islet and papillary nucleus. Conclusion Thiopental sodium anesthesia has a clear site of action in the central nervous system.