用平衡透析法研究了等电点时Cu(Ⅱ )与人血清白蛋白 (HSA)或牛血清白蛋白 (BSA)的相互作用 Scatchard图分析表明 ,HSA和BSA对Cu(Ⅱ )各有 1个强结合部位 ,它可能位于白蛋白分子的N 端三肽段上 为全面反映Cu(Ⅱ )与HSA或BSA的结合情况 ,用非线性最小二乘法拟合Bjerrum方程 ,首次报道了等电点时Cu(Ⅱ ) HSA和Cu(Ⅱ ) BSA体系的逐级稳定常数值 ,其K1的数量级为 10 4 Hill常数分析表明Cu(Ⅱ )与HSA或BSA的结合均产生了一定的正协同效应 The interaction of Cu (Ⅱ) with human serum albumin (HSA) or bovine serum albumin (BSA) at isoelectric point was studied by equilibrium dialysis. Scatchard analysis showed that HSA and BSA have one Strong binding sites, which may be located on the N-terminal tripeptide of albumin molecules, fully reflect the binding of Cu (Ⅱ) to HSA or BSA. The Bjerrum equation is fitted by a nonlinear least-squares method and the isoelectric point The step-by-step stability constants of Cu (Ⅱ) HSA and Cu (Ⅱ) BSA systems, with K1 orders of magnitude 10 4 Hill constant analysis, showed that there was some positive synergistic effect between Cu (Ⅱ) and HSA or BSA