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从天津近郊番茄上获得烟草花叶病毒(TMV)分离物58份,从中选出10个代表性的分离物为抗原与10个TMV单克隆抗体开展ELISA间接法试验,并分别接到10个鉴别寄主上,按症状合并归为六类,结果从10个分离物中初步识别出五个抗原型和抗原决定簇.其中四个抗原型和四个症状类别的成员完全相符,一个抗原型的两个
Fifty-eight tobacco mosaic virus (TMV) isolates were obtained from the outskirts of Tianjin. Ten representative isolates were selected for ELISA indirect ELISA and 10 differential anti-TMV monoclonal antibodies The host, according to the symptoms merged into six categories, the results of 10 isolates initially identified five antigens and antigenic determinants.Among them four antigens and four symptom categories of members exactly the same, an antigen-based two A