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以3个甜瓜品种“BM7”、“伽师瓜”和“Y8”为试材,选取甜瓜基因组的3个DNA区域进行SSR位点的分布规律分析,进一步通过聚丙烯酰胺凝胶电泳检验甜瓜基因组SSR位点的多态性,以期初步揭示甜瓜基因组中SSR位点的分布规律及其多态性,进而为甜瓜及其它物种开展相关研究提供参考。试验表明:甜瓜基因组中平均每1.9kb就有一个SSR位点,富含A和T的重复基序出现的频率远高于富含C和G的重复基序的频率,没有发现全部由C、G构成的重复基序;二、三、四碱基重复基序的SSR位点占绝大多数(83.3%),五、六碱基重复基序的SSR位点分布较少(16.7%)。二、三、四、五、六核苷酸重复基序SSR位点平均长度依次为21、15、14、16、20bp。设计的82对SSR引物中81对扩增出产物,多态比例最高为48.1%。试验表明,甜瓜基因组中SSR分布具有一定规律,并且可以利用SSR分子标记开展相关研究;试验同时对更好地开展SSR和ISSR分子标记的相关研究奠定了理论基础和依据。
The distribution patterns of SSR loci in three melon cultivars “BM7 ”, “Jiashi melon” and “Y8 ” were studied. The distribution of three SSR loci was analyzed by polyacrylamide The polymorphism of SSR loci in melon genome was detected by gel electrophoresis in order to reveal the distribution and polymorphism of SSR loci in melon genome and provide reference for related studies on melon and other species. The results showed that there was one SSR site per 1.9kb in melon genome. The frequency of repeat motifs rich in A and T was much higher than that of repeat motifs rich in C and G, G, SSR loci of the second, third and fourth base repeat motifs accounted for the vast majority (83.3%), SSR loci of five and six base repeat motifs were less distributed (16.7%). The average lengths of SSR loci in the two, three, four, five and six nucleotide repeat motifs were 21, 15, 14, 16 and 20 bp. Eighty-two pairs of SSR primers were designed to amplify the products, with the highest percentage of polymorphic bands being 48.1%. The results showed that the distribution of SSR in melon genome had certain rules and SSR markers could be used to carry out related research. The study also laid a theoretical foundation and basis for better research on SSR and ISSR molecular markers.