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目的:探讨不同的中药复方对大鼠脑缺血再灌注血浆凝血与纤溶相关指标血管性血友病因子(vWF)、组织型纤溶酶原激活物(t-PA)和组织型纤溶酶原激活物抑制物(PAI-1)的动态影响。方法:采用线栓法制作大鼠局灶性脑缺血再灌注模型,随机分为假手术组、模型组、益气活血方组、镇肝熄风汤组和星蒌承气汤组,分别给予相应药物灌胃;采用酶联免疫法检测血浆vWF、tPA和PAI-1含量。结果:模型组再灌注各时间点血浆vWF、t-PA含量显著升高,PAI-1血浆含量显著下降;各治疗组再灌注各时间点血浆vWF、t-PA含量显著降低和PAI-1含量显著升高;在降低vWF方面,24 h潜阳熄风组优于化痰通腑组和益气活血组,48 h化痰通腑组优于潜阳熄风组和益气活血组,72 h益气活血组优于潜阳熄风组和化痰通腑组;在降低血浆t-PA,24 h化痰通腑组优于潜阳熄风组和益气活血组,72 h各治疗组组间比较益气活血组优于化痰通腑组和潜阳熄风组;在升高PAI-1方面,72 h各组间比较益气活血组优于化痰通腑组和潜阳熄风组。结论:不同的中药复方可以降低vWF、t-PA血浆含量和升高PAI-1血浆含量抗脑缺血再灌注损伤,72 h益气活血方作用效果最佳。
OBJECTIVE: To investigate the effects of different Chinese herbal compounds on vWF, t-PA and tissue fibrinolysis in rats with cerebral ischemia-reperfusion injury, Dynamic effects of zymogen activator inhibitor (PAI-1). Methods: The model of focal cerebral ischemia-reperfusion in rats was made by thread plug method and randomly divided into sham operation group, model group, Yiqi Huoxue Fang group, Zhengan Xifeng Decoction group and Xinglou Chengqi Decoction group, respectively The corresponding drugs were given intragastrically. The levels of vWF, tPA and PAI-1 in plasma were detected by enzyme-linked immunosorbent assay. Results: The content of plasma vWF and t-PA in model group increased significantly and the plasma level of PAI-1 decreased significantly at each time point after reperfusion. The content of vWF and t-PA in plasma and the content of PAI-1 (P <0.05), and the level of vWF was lower than that of the control group (P <0.05) h was better than that of the yang-qi-huoxue-treated group and the Huatan-tong-fu-qing group. In reducing plasma t-PA, The comparison of Yiqi Huoxue group was better than Huatan Tongfu group and Qianyangxifeng group; in terms of increasing PAI-1, the comparison of Yiqihuoxue group at 72h was better than Huatan Tongfu group and Qianyang group Xifeng group. Conclusion: Different traditional Chinese medicine compounds can reduce the vWF, t-PA plasma content and increase the plasma PAI-1 anti-cerebral ischemia-reperfusion injury, 72 h Yiqihuoxue prescription effect best.