论文部分内容阅读
血液中安替比林的测定是鉴定肝功能损伤的一种生物学指标。高压液相色谱(HPLC)外标法具有较内标法和紫外分光光度法(uv)更简便、快速,需血量少(0.1ml)等特点。灵敏度为1 μg/ml。适于大量样品的测定,可在临床上推广使用。 一、实验方法 1.仪器:Waters公司HPLC 490型多波长分光光度检测器;色谱柱:μ-Bondpak C18(25cm×3mm):柱前加Guard-pak预柱。 2.HPLC条件,①流动相:甲醇0.6ml/min,水0.5ml/min,泵压约119kg/cm2;②波长:254nm。 3.操作步骤:①标准曲线的绘制:将肝素化
Determination of antipyrine in blood is a biological indicator of liver damage. High pressure liquid chromatography (HPLC) external standard method with internal standard method and ultraviolet spectrophotometry (uv) more simple, fast, less blood (0.1ml) and so on. Sensitivity of 1 μg / ml. Suitable for the determination of a large number of samples, can be widely used in clinical practice. First, the experimental method 1. Instruments: Waters 490 wavelength multi-wavelength spectrophotometer detector; Column: μ-Bondpak C18 (25cm × 3mm): Pre-column Guard-pak pre-column. 2. HPLC conditions, ① mobile phase: methanol 0.6ml / min, water 0.5ml / min, pump pressure of about 119kg / cm2; ② wavelength: 254nm. 3 steps: ① standard curve of the drawing: the heparin