目的建立小鼠不同部位黑色素瘤模型，为不同研究目的提供合适的动物肿瘤模型。方法接种B16小鼠黑色素瘤细胞于BALB／C小鼠足部、腹腔和尾静脉，建立移植瘤模型和肺部转移瘤模型。观察B16实体瘤的形态学特点，接种细胞数与肿瘤生成的关系，肿瘤生长对小鼠生存时间、血清肿瘤坏死因子（sTNF）和唾液酸（SA）的影响。结果足部接种5X10瘤细胞后10天，肿瘤进入对数生长，成瘤率近100％；腹腔接种瘤细胞数大于5000个／只，成瘤率与接种细胞数成正比；接种3X10细胞／只，10天后在小鼠腹壁和肠系膜可见散在黑色素瘤结节，荷瘤鼠sTNF和SA水平明显高于正常小鼠，且与肿瘤的大小和数量成正比，小鼠平均存活59天；尾静脉接种后3周，肺部可见大小不等转移瘤灶，两肺平均瘤灶数可达192.11士92。结论B16黑色素瘤细胞株在BALB／C小白鼠具有很高的成瘤率和转移特性，且较C57／B16小黑鼠模型具有方便、稳定和观察容易等优点，是一较理想的模型。 Objective To establish a mouse model of melanoma in different sites and provide suitable animal tumor models for different research purposes. METHODS: B16 mouse melanoma cells were inoculated into the foot, peritoneal cavity, and tail vein of BALB/C mice to establish a transplanted tumor model and a metastatic tumor model. Observe the morphological characteristics of B16 solid tumors, the relationship between the number of inoculated cells and tumor growth, the effect of tumor growth on the survival time of mice, serum tumor necrosis factor (sTNF) and sialic acid (SA). Results 10 days after inoculation of 5X10 tumor cells in the foot, the tumors entered logarithmic growth, the tumorigenesis rate was nearly 100%; the number of inoculated tumor cells in the abdominal cavity was greater than 5,000 per mouse; the tumor formation rate was directly proportional to the number of inoculated cells; 3X10 cells were inoculated/only 10 days later, scattered melanoma nodules were observed in the abdominal wall and mesentery of mice. The levels of sTNF and SA in tumor-bearing mice were significantly higher than those in normal mice and were proportional to the size and number of tumors. The mice survived on average for 59 days; tail vein inoculation Three weeks later, the lungs showed metastatic tumors of varying sizes. The average number of tumors in both lungs was as high as 192.11±92. Conclusion The B16 melanoma cell line has high tumorigenicity and metastasis characteristics in BALB/C mice, and it is a more ideal model than the C57/B16 black rat model for its convenience, stability and easy observation.