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目的:观察针对HBV C 区双位点核酶对2 .2 .15 细胞中HBEAG 表达的抑制作用.方法:利用亚克隆技术,从质粒P GEM － RZ123 切下ECOR I－ BAM H I 片段克隆于真核表达质粒PBBS212 中,将该质粒转染2 .2 .15 细胞,用ELISA 及免疫组化方法观察该核酶对HBEAG 合成的抑制作用.结果:细胞中表达出针对HBV C 区核酶,该核酶对HBEAG 的抑制率达48 .6 ％ .结论:该双位点核酶可能通过针对HBV C 区基因的剪切作用,阻断C 区基因的表达“,”jective:To observe the inhibition of HBeAg expression in the 2.2.15 cell transfected by two-locus ribozyme against HBV.Methods:The two-locus ribozyme gene, cut from plasmid pGEM-Rz123, was cloned into the eukaryotic expression vector pBBS212. The recombinant plasmid pBBS212-Rz was transfected into 2.2.15 cell using lipofectamine. The supernate antigen of HBV was detected by ELISA and immunohistochemistry to clarify the inhibition of HBeAg.Results:Ribozyme could be expressed in 2.2.15 cell by in-situ hybridization.Meanwhile,this two-locus ribozyme could reduce the level of HBeAg expression in 2.2.15 cell by up to 48.6%.Conclusions:The two-locus ribozyme can inhibit the expression of HBeAg in transfected 2.2.15 cell by cutting the relative HBV gene.