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目的探讨SO2吸入对运动大鼠心肌胶原纤维形态及胶原蛋白含量的影响。方法 24只雄性SD大鼠随机分为空白对照组、单纯运动组、单纯SO2污染组、SO2污染运动组,每组6只。单纯运动组、空白对照组大鼠置于自然环境中,单纯SO2污染组、SO2污染+运动组置于人工模拟的SO2污染环境中(10 mg/m3,1 h/d,连续4周),单纯运动组、SO2污染+运动组大鼠进行跑轮运动(8 m/min,1 h/d,连续4周)。采用胃酶酸解法测定大鼠心肌羟脯氨酸的含量,并计算胶原浓度。采用苦味酸-天狼猩红染色法检测大鼠心肌、小动脉周围胶原容积分数。结果单纯运动组、单纯SO2污染组和SO2污染+运动组大鼠心肌胶原纤维较空白对照组增生,且SO2污染+运动组大鼠心肌胶原纤维过度堆积。SO2污染+运动组、单纯SO2污染组、单纯运动组大鼠心肌羟脯氨酸含量、胶原浓度较空白对照组升高(P<0.05),SO2污染+运动组升高更明显(P<0.01);单纯SO2污染组、SO2污染+运动组心肌、小动脉血管周围胶原容积分数较空白对照组大鼠升高(P<0.05),SO2污染+运动组大鼠升高更明显(P<0.01)。结论 SO2吸入和运动双重刺激作用下,大鼠心肌胶原纤维过度增生,胶原蛋白合成与降解失衡,从而影响心肌细胞间力的传递及心脏的正常舒缩功能。
Objective To investigate the effects of SO2 inhalation on cardiac collagen fibers and collagen content in exercise-induced rats. Methods Twenty-four male SD rats were randomly divided into blank control group, simple exercise group, pure SO2-contaminated group and SO2-contaminated exercise group, with 6 rats in each group. The pure exercise group and blank control group were placed in the natural environment, SO2 pollution alone group and SO2 pollution + exercise group were placed in artificial simulated SO2 pollution environment (10 mg / m3, 1 h / d for 4 weeks) Simple exercise group, SO2 pollution + exercise group rats exercise (8 m / min, 1 h / d, for 4 weeks). The content of hydroxyproline in rat myocardium was determined by gastric acidolytic method and the collagen concentration was calculated. The contents of collagen in myocardium and small artery of rats were measured by picric acid-Sirius red staining. Results Compared with the control group, the myocardial collagen fibers in pure exercise group, SO2-contaminated group and SO2-contaminated + exercise group proliferated more than blank control group, and the myocardial collagen fibers were over-accumulated in SO2-contaminated + exercise group. Compared with the blank control group, the content of hydroxyproline and the concentration of collagen in SO2-contaminated + exercise group, SO2-contaminated group and pure exercise group increased significantly (P <0.05), SO2 + ); SO 2 pollution + exercise group myocardial collagen content in arterial and perivascular arteries increased significantly (P <0.05), SO 2 pollution + exercise group rats increased more significantly (P <0.01 ). Conclusion SO2 inhalation and exercise double stimulation, myocardial collagen fibers hyperplasia, collagen synthesis and degradation of imbalance, thereby affecting the transmission of cardiac muscle cells and cardiac systolic and diastolic function.