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106只小鼠随机分成5组,每组18至23只。1～3组小鼠皮下注射1,2-二甲基肼(DMH),剂量20mg/kg体重,每周一次,连续20周。实验结束前,动物分别自由饮用自来水(第一组),水加嗜酸乳酸杆菌酸牛乳(第二组)及水加牛乳(第三组)。第4组和第5组为对照组,动物分别皮下注射1mM EDTA和蒸馏水。 27周诱癌期后,对照组小鼠未发生肿瘤。DMH处理动物中,第2组的结肠癌发生率为44.4％,比第1组(80％)和第3组(100％)明显降低(P<0.05)。此外,实验期间第2组动物的存活率(100％) 比第1组(80％)和第3组(74％)都高。结论是喂饲嗜酸乳酸杆菌(Shahani株)制备的酸牛乳能显著降低DMH诱发的小鼠癌肿和死亡率,而单纯牛乳则能增加。本研究的资料提示,食物中添加活嗜酸乳酸杆菌可能有助于预防结肠癌。
106 mice were randomly divided into 5 groups of 18 to 23 mice each. Groups 1 to 3 mice were injected subcutaneously with 1,2-dimethylhydrazine (DMH) at a dose of 20 mg / kg body weight once a week for 20 weeks. Before the end of the experiment, the animals were given free access to tap water (group 1), water plus Lactobacillus acidophilus milk (group 2), and water plus milk (group 3). Groups 4 and 5 were control animals, animals were injected subcutaneously with 1 mM EDTA and distilled water, respectively. Twenty-seven weeks after induction of cancer, mice in the control group did not develop tumors. Among DMH-treated animals, the incidence of colon cancer in Group 2 was 44.4%, significantly lower than Group 1 (80%) and Group 3 (100%) (P <0.05). In addition, the survival rate of Group 2 animals during the experiment (100%) was higher than that of Group 1 (80%) and Group 3 (74%). The conclusion is that the yoghurt prepared by feeding Lactobacillus acidophilus (Shahani strain) can significantly reduce the cancer-causing and mortality rate of DMH-induced mice, while the milk yield of pure milk can be increased. The data in this study suggest that adding Lactobacillus acidophilus to food may help prevent colon cancer.