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A 4.34 liter two stage air lift photobioreactor incorporating Anabaena variabilis ATCC29413 mutant PK84 was used to study H 2 production. Results showed that H 2 production increased with increasing light intensity from 47 μE/(m 2·s) up to 190 μE/(m 2·s), but that further increase of light intensity decreased the H 2 production because of the inhibition due to the high pO 2. The data also indicated that longer argon gas charge resulted in more H 2 produced due to the increase of nitrogenase activities and heterocyst frequency, and that more than 1.3 L net H 2 was produced from this computer controlled photobioreactor.
A 4.34 liter two stage air lift photobioreactor incorporating Anabaena variabilis ATCC29413 mutant PK84 was used to study H 2 production. Results showed that H 2 production increased with increasing light intensity from 47 μE / (m 2 · s) up to 190 μE / (m 2 · s), but that further increase of light intensity decreased the H 2 production because of the inhibition due to the high pO 2. The data also indicates that longer argon gas charge resulted in more generated than 2 and heterocyst frequency, and that more than 1.3 L net H 2 was produced from this computer controlled photobioreactor.