水稻(Oryza sativa L.)苗期低温白化突变体al12的超微结构与基因定位

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水稻苗期低温白化突变是水稻在发育早期对低温胁迫的一种适应性,是一种受发育和温度控制的条件表达,它与其他水稻白化突变有本质的不同.本研究利用便携式叶绿素测量仪测定了白化时期植株的叶绿素含量和用透射电镜观察了叶绿体的结构变化.结果发现叶绿素平均含量仅为1.2(SPAD),而叶绿体也不能正常发育仅有囊泡状结构.通过与9311的正反交实验及子代的分离表现证明该性状受一个隐性核基因的控制.另外利用SSR分子标记技术将该基因定位在第8染色体上,两侧最近的SSR标记RM5068和RM3702分别距基因0.5~1.1 cM和4.9 cM,基因被定位在约6个cM的区间内.我们将该基因暂时命名为al12.
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