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摘要目的探讨影像引导下进行肿块中心穿刺活检所获取核酸数量与质量的变量和影响因素。材料与方法本研究由机构伦理委员会批准并符合HIPAA。前瞻性地收集了74例病人的232个活检标本(包括49例病人手术切除肿块的177个离体穿刺的标本和25例病人CT引导下肺活检的55个活体肺穿刺标本),并就穿刺针规格(gauge)、穿刺针数和穿刺针芯的百分比来定量测量DNA和RNA量。RNA质量也要评估。采用方差分析和线性回归分析评估变量之间相关
Abstract Objective To investigate the variables and influencing factors of the number and quality of nucleic acids obtained by biopsy of the mass guided by imaging. Materials and Methods The study was approved by the Institutional Ethics Committee and conforms to HIPAA. A total of 232 biopsy specimens of 74 patients (including 177 biopsied specimens of 49 patients with surgically resected masses and 55 live-lung specimens of CT-guided lung biopsy from 25 patients) were collected prospectively and punctured Needle gauge, the number of needles and the percentage of puncture needle core to quantitatively measure the amount of DNA and RNA. RNA quality is also assessed. Variance analysis and linear regression analysis were used to assess the correlation between variables