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目的:探讨肿瘤坏死因子-β(tumor necrosis factor-β,TNF-β)rs1041981位点基因多态性与南京地区老年冠状动脉粥样硬化性心脏病(coronary artery disease,CAD)遗传易感性的关系。方法:采用病例对照研究方法,选择确诊的399例CAD患者和280例健康对照者,应用高通量TaqMan-MGB探针技术对TNF-βrs1041981位点进行基因分型,同时采用荧光定量PCR法对其进行检测。结果:CAD组TNF-βrs1041981基因型分布(CC型25.1%、CA型50.3%、AA型24.6%)与对照组(41.1%、41.8%、17.1%)的差异具有统计学意义(χ2=20.1,P<0.05);基因型频率的相对风险分析发现,AA型及CA型患CAD的风险是CC型的1.38倍(OR=1.38,95%CI:0.84～2.05);CAD组TNF-β及超敏C反应蛋白血清水平[(38.74±16.42)pg/ml,(6.22±3.74)mg/L]显著高于对照组[(12.26±10.37)pg/ml,(2.37±1.17)mg/L,P<0.05]。结论:TNF-βrs1041981基因多态性与本地区CAD的发病有关,等位基因A可能是CAD发生的重要易感基因。
Objective: To investigate the relationship between gene polymorphism of rs1041981 in tumor necrosis factor-β (TNF-β) and genetic susceptibility to coronary artery disease (CAD) in Nanjing. . Methods: A case-control study was conducted in 399 CAD patients and 280 healthy controls. The high-throughput TaqMan-MGB probe technique was used to genotype TNF-β rs1041981. Fluorescent quantitative PCR It is tested. Results: The genotype distribution of TNF-βrs1041981 in CAD group was statistically significant (χ2 = 20.1, 41.8%, 17.1%) with CC type 25.1%, CA type 50.3%, AA type 24.6% (OR = 1.38, 95% CI: 0.84 ~ 2.05); CAD group TNF-β and super (P <0.05); the relative risk analysis of genotype frequencies found that the risk of type CAD CAD and CA CAD was 1.38 times Serum C-reactive protein level was significantly higher than that of the control group [(38.74 ± 16.42) pg / ml, (6.22 ± 3.74) mg / L, <0.05]. CONCLUSION: TNF-β rs1041981 gene polymorphism is associated with the development of CAD in the region. Allele A may be an important predisposing gene for CAD.