对孢子丝菌病的诊断可做直接涂片或组织病理切片检查,有时见菌丝或孢子,但阳性率甚低,对其确切诊断还有待于真菌培养。做真菌培养怎样避免不必要的污染,如何采取标本较为恰当,确有探讨必要。因皮损的形态不同,分别应用以下方法采取标本。(一)当皮损为溃疡时,以生理盐水将脓性分泌物洗净,露出清洁的肉芽组织,用接种环在靠近皮肤的肉芽处,稍许伸入组织内 The diagnosis of sporotrichosis can be done directly smear or histopathological examination, and sometimes see mycelium or spores, but the positive rate is very low, its exact diagnosis remains to be fungal culture. How to do fungus culture to avoid unnecessary pollution, how to take specimens more appropriate, there is indeed a need to explore. Due to the different morphological lesions, the following methods were used to take specimens. (A) when the lesion is ulcer, the purulent secretions washed with saline to reveal clean granulation tissue, inoculation ring in the granulation near the skin office, slightly into the tissue