人腺病毒基因早期转录区4的第4开放阅读框架编码的E4 ORF4蛋白是一种多功能病毒调节蛋白,能够特异地引起转化细胞的凋亡,而对正常细胞没有影响,并且这种凋亡机制不依赖于经典的p53途径。E4 ORF4蛋白功能的发挥需要与细胞内蛋白磷酸酶2A的相互作用,而且它还能改变Src激酶的活性。E4 ORF4蛋白的多种功能协调合作,最终导致了转化细胞的凋亡。 The E4 ORF4 protein encoded by the 4th open reading frame of human adenovirus early transcription region 4 is a multifunctional viral regulatory protein that specifically induces apoptosis in transformed cells without affecting normal cells and this apoptosis The mechanism does not depend on the classical p53 pathway. The function of the E4 ORF4 protein requires interaction with the intracellular protein phosphatase 2A, and it also changes Src kinase activity. The coordinated functioning of multiple functions of the E4 ORF4 protein eventually led to the apoptosis of transformed cells.