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为筛选组织培养特性优良的大麦品种并建立高效的再生体系,选用西北地区主栽品种甘啤3号、甘啤4号、甘啤6号及德国引进品种玛俐、博乐共5个大麦品种,分别研究了基因型、培养基成分、种子处理方式等因素对大麦成熟胚组织培养的影响。结果表明,不同大麦品种的出愈率、绿点率和绿苗率均存在极显著差异,其中博乐的综合效应最好。脱分化培养基A3、分化培养基C2是适合进行再生体系构建的培养基。胚刮碎与纵切处理的愈伤组织诱导率显著高于横切。甘啤3号、甘啤4号、博乐的愈伤组织诱导频率和分化绿苗率均较高,是适合进行遗传转化的受体材料。
In order to screen tissue culture characteristics of barley varieties and establish an efficient regeneration system, select the main cultivars in Northwest China such as Gan Beer 3, Ganpi 4, Ganpi 6 and the introduction of German varieties Mali, Bole total 5 barley varieties The effects of genotype, medium composition and seed treatment on the tissue culture of mature barley were studied. The results showed that there were extremely significant differences among different barley cultivars in culling rate, green point rate and green shoot rate, of which Bole was the best. Dedifferentiation medium A3 and differentiation medium C2 are suitable mediums for regeneration system construction. Embryo scraping and slitting treatment callus induction rate was significantly higher than the cross. Ganpi 3, Ganpi 4, Bole callus induction frequency and differentiation of green shoot rate are high, is suitable for genetic transformation of the receptor material.