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选用一对免疫球蛋白重链基因V区和J区的通用引物,进行PCR检测不同类型小儿急性白血病免疫球蛋重链基因重排。33例小儿急性白血病初诊未治骨髓标本检测结果呈单克隆条带的为U-ALL3/7例,C-ALL4/6例,单/B祖双标记白血病1/3例,T-ALL1/4例,AML1/10例。B-ALL、AMoL、髓/T双标记白血病各1例均阴性。说明免疫球蛋白重链基因重排主要见于B细胞系ALL及其与B细胞相关的双标记白血病,阳性率为47％,可以作为分析白血病细胞来源的标志之一,并辅佐基因分型。另外对20例正常人外周血单个核细胞DNA检测结果均为一片模糊,无明确条带,说明可以区分淋巴细胞系克隆增殖和反应性增生。本文尚对2例PCR产物进行DNA序列分析,结果参与重排的J区分别为J_4和J_5,DNA同源性很小,有可能设计克隆特异性引物和探针作微小残留病检测。
A pair of universal primers of immunoglobulin heavy chain gene V region and J region were selected for PCR to detect immunoglobulin heavy chain gene rearrangements of different types of children with acute leukemia. A total of 33 cases of acute leukemia in children with newly diagnosed untreated bone marrow specimens showed monoclonal bands for the U-ALL3 / 7 cases, C-ALL4 / 6 cases, single / B progenitor double labeled leukemia in 1/3 cases, T-ALL1 / 4 For example, AML1 / 10 cases. B-ALL, AMoL, marrow / T double-labeled leukemia in 1 case were negative. Immunoglobulin heavy chain gene rearrangement is mainly found in B cell line ALL and B cell-associated double-marker leukemia, the positive rate was 47%, can be used as a marker of leukemia cells, and assisted genotyping. In addition, the results of DNA test of peripheral blood mononuclear cells in 20 normal persons were all fuzzy, with no clear bands, indicating that the clonal proliferation and reactive hyperplasia of lymphocyte lines could be distinguished. In this paper, DNA sequence analysis of two PCR products was performed. The results showed that the J regions involved in rearrangement were J_4 and J_5, respectively. DNA homology was small, and it was possible to design cloning specific primers and probes for the detection of minimal residual disease.