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目的:探讨鼻咽癌细胞中EBV编码的miR-BHRF1-1在病毒复制中的作用。方法:在鼻咽癌细胞中,分别在或不在抑制miR-BHRF1-1的情况下以佛波酯(TPA)诱导病毒复制或者过表达miR-BHRF1-1,检测病毒复制指标BZLF1、Ea-D和BamHI-W片段以及细胞p53蛋白和mRNA的变化。通过在荧光素酶mRNA3-非
AIM: To investigate the role of EBV-encoded miR-BHRF1-1 in viral replication in nasopharyngeal carcinoma cells. Methods: In nasopharyngeal carcinoma cells, virus replication or overexpression of miR-BHRF1-1 was induced by phorbol ester (TPA) with or without inhibition of miR-BHRF1-1, and the viral replication markers BZLF1 and Ea-D And BamHI-W fragments as well as cellular p53 protein and mRNA changes. Through luciferase mRNA3-non