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目的 :探索缺血预处理对肢体再灌注损伤的保护作用。方法 :分别给予兔后肢一次或多次短暂的缺血预处理 ,然后于持续缺血 4h ,再灌注 1h后行股静脉穿刺 ,抽血检测血清肌磷酸激酶 (CPK)、谷草转氨酶 (GOT)、乳酸脱氢酶 (LDH)及超氧化物歧化酶 (SOD)和丙二醛 (MDA)含量 ,并取腓肠肌病检。结果 :缺血预处理各组再灌注后肌细胞内酶释放减少 ,病理切片也发现骨骼肌坏死和梗塞灶减少 ,血清MDA含量下降 ,而SOD活力升高。不同次数的缺血预处理结果之间存在差异。结论 :缺血预处理可以影响体内的自由基代谢 ,对肢体再灌注损伤具有保护作用 ,且这种保护作用与预处理次数有一定关系。
Objective: To explore the protective effect of ischemic preconditioning on limb reperfusion injury. Methods: One or more short-term ischemic preconditioning were given to the hind limbs of rabbits respectively. Then, the perfusion of femoral vein was performed 4h after reperfusion and 1h after reperfusion. Blood samples were collected for determination of serum phosphokinase (CPK), aspartate aminotransferase (GOT) Lactate dehydrogenase (LDH) and superoxide dismutase (SOD) and malondialdehyde (MDA) content, and taken gastrocnemius disease examination. Results: After ischemia-reperfusion, the intracellular enzyme release decreased after ischemia-reperfusion. Skeletal muscle necrosis and infarction were also found in the pathological sections. The content of serum MDA decreased and the activity of SOD increased. There are differences between different times of ischemic preconditioning results. CONCLUSION: Ischemic preconditioning can affect free radical metabolism in the body and protect limbs against reperfusion injury, and this protective effect is related to the number of pretreatment.