感受态大肠杆菌细胞的制作和转化是分子生物学领域基因工程中最常用和最基本的技术。目前主要采用电转化法 ,Ｈａｎａｈａｎ改良法和Ｃｏｈｅｎ改良法[1] 。电转化法效率高 ,但需要特殊的基因导入仪器 ,而Ｈａｎａｈａｎ法和Ｃｏｈｅｎ法操作比较繁琐。笔者在实践中摸索出适应 The production and transformation of competent E. coli cells is the most common and fundamental technique in genetic engineering in molecular biology. Currently mainly used electrotransformation, Hanahan improved method and Cohen improved method [1]. The electrotransformation method is highly efficient, but requires special gene introduction instruments, whereas the Hanahan and Cohen methods are cumbersome. I find out in practice adaptation