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目的探索一种快捷、简便的大脑锥体细胞和小脑普肯耶细胞的染色方法。方法取猫、家兔、大鼠和豚鼠的大脑和小脑组织,升汞-重铬酸钾-铬酸钾固定液固定、浸染后,部分组织按传统的Cox法制片,即固定后进行火棉胶包埋、滑式切片机切片;部分组织用改良法制片,即组织不经包埋而直接进行振荡切片。结果与传统Cox法相比,应用振荡切片的Cox法减少了操作程序,大大缩短了制片时间,神经元染色均匀,神经元突起更为完整,小突起细节显示更为满意,且背景浅淡,非特异性沉淀颗粒极少。结论应用振荡切片的Cox改良法快捷、简便,神经元染色效果明显优于传统Cox法。
Objective To explore a fast and simple method for staining cerebellum and cerebellum Purcomm cells. Methods The brain and cerebellum of cats, rabbits, rats and guinea pigs were fixed with mercuric chloride-potassium dichromate-potassium chromate solution. After soaking, some tissues were prepared according to the traditional Cox method. Plastic embedded, slides slide section; some organizations to improve the legal system, that is, the tissue without embedding and direct oscillation slice. Results Compared with the traditional Cox method, the Cox method using the oscillatory slice reduced the operation procedure, greatly shortened the preparation time, even stained neurons, more complete neurite protuberances, more detail of small protuberances, Non-specific precipitation of particles is minimal. Conclusion The Cox modified method using oscillatory slices is quick and easy, and the staining effect of neurons is obviously better than the traditional Cox method.