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作者报告建立和应用包括单克隆抗体结合免疫荧光、蛋白免疫印迹、DNA同源性杂交、DNA酶切图谱、脂肪酸气-质谱及免疫电镜在内的一套分子立克次体学方法,对我国蜱传斑点热立克次体病进行了分子流行病学研究。结果:(1)证明在新疆精河县、内蒙呼盟陈旗及北京昌平县存在北亚蜱传斑点热(北亚热)的自然疫源地;(2)在我国首次从北亚热病人中分离出西伯利亚立克次体(An-84,Se-85、W-88 3株),从草原革蜱(FT-84、MT-84 2株),中华革蜱(BJ-90 1株),草原革蜱蜱卵(TO-85 1株)也分离出同一性质立克次体,自易感人群、传播媒介和传染源3个环节中都找到了病原学证据;(3)在种的水平上研究了上述7株分离株和1株国内参考株(草原革蜱JH-74株),在分类学上确定了它们均属立克次体属、斑点热群、西伯利亚立克次体种(Rickettsia sibirica)。在我国东经80～135°,北纬40～50°广大地理范围内,用分子流行病学方法,确证和新发现了一个病种——北亚蜱传斑点热(North Asia Tick Borne Spotted Fever)。
The authors report the establishment and application of a set of molecular Rickettsia science methods including monoclonal antibody binding immunofluorescence, western blotting, DNA homology hybridization, DNA digestion mapping, fatty acid gas-mass spectrometry and immunoelectron microscopy, Tickspecies hot rickettsial disease molecular epidemiology. Results: (1) It is proved that there exists a natural foci of northern tick-blotch fever (NEA) in Jinghe County, Inner Mongolia, Huimin Chenqi, Inner Mongolia and Changping County, Beijing; (2) (3 strains of An-84, Se-85 and W-88) were isolated from the genus Siberian rickettsiae (FT-84, MT-84 2) (TO-85 1 strain) also isolated rickettsiae of the same nature, and found etiological evidence from the three links of susceptible population, vector and source of infection; (3) The above seven isolates and one domestic reference strain (JH-74 strain) were studied horizontally, and they were classified as Rickettsia, Spotted fever, Siberian rickettsia (Rickettsia sibirica). A molecular species epidemic, North Asia Tick Borne Spotted Fever, was confirmed and newly discovered in our country with a longitude of 80 ~ 135 ° E and 40 ~ 50 ° N.