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当前应用“5406”孢子粉直接堆制菌肥正在广泛推广。为了解决生产队生产“5406”孢子粉工作中菌种用量大,生产周转不开的矛盾,我们试验了用孢子粉生产孢子粉的连续生产方法,获得成功。这种方法简单易行,省略了常法生产中逐级生产菌种的繁琐工序;便于集中生产,供直接堆制应用,可以大大节省人力、物力和燃料。 “5406”孢子粉连续生产,首先选择固体培养中生长好的孢子粉两瓶放入接种箱内,加上500毫升无菌水,充分摇匀,制成悬液;再接种于消毒好的固体培养基瓶中(配料同固体培养)。一瓶悬液可接80—100瓶。然后放在30—32℃下培养。其他发酵管理方法与固体培养阶段相同。这样循环接种生产5—6批后,再用常法生产菌种连续生产。用这种方法生产的孢子数一般均可达到标准。
The current application of “5406” spore powder direct compost fertilizer is widely promoted. In order to solve the conflict between the production of “5406” spore powder in the production team and the production turnover is not open, we tested the continuous production of spore powder production with spore powder, and achieved success. This method is simple and easy, omitting the tedious process of producing strains step by step in the normal production method, facilitating the centralized production for direct stacking application, which can greatly save manpower, material resources and fuel. “5406” spore powder continuous production, first of all, select the solid culture spore powder grow two bottles into the inoculation box, add 500 ml of sterile water, shake well to make a suspension; re-inoculation of sterilized solid Medium bottle (ingredients with solid culture). A bottle of suspension can be 80-100 bottles. Then incubated at 30-32 ° C. Other fermentation management methods are the same as for the solid culture phase. This cycle of inoculation production 5-6 batches, and then continue production of bacteria often produced. The number of spores produced by this method can generally reach the standard.