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通过农杆菌介导法将提高植物抗寒能力的水稻OsDREB基因导入芦荟,共培养后的芦荟外植体在含G418筛选剂的MS培养基上经多次筛选和分化,获得了一定数量的抗性再生芽,对移载成活的抗性再生植株进行PCR检测。表明OsDREB基因已经导入芦荟基因组中,转化率为0.5%。采用电导率测定的方法检测转化DREB基因对芦荟抗逆性的影响,以低温逆境对转化DREB基因得到的芦荟再生植株进行胁迫处理,同时观察转基因植株的生长发育和抗性变化,发现对照芦荟植株的叶片显褐色干枯半透明,出现严重冻伤的迹象,而转基因植株生长情况较好,且转基因植株的相对电导率与对照植株相比明显较低。结果表明外源基因已成功整合入芦荟基因组DNA中并得到稳定表达。
The rice OsDREB gene that enhances the plant’s cold resistance was introduced into aloe by agrobacterium-mediated method. The cocultivated aloe explants were screened and differentiated on MS medium containing G418 screening agent for a number of times to obtain a certain amount of anti- The regenerated shoots were tested for PCR against live, resistant, regenerated plants. OsDREB gene has been introduced into the genome of aloe, the conversion rate of 0.5%. The effect of transformed DREB gene on the resistance of aloe to transformation was tested by conductivity measurement. The aloe regeneration plants transformed with DREB gene were stressed under low temperature stress. The growth and development of transgenic plants and the changes of resistance were also observed. The leaves were brown dry and semitransparent, showing signs of severe frostbite, while the transgenic plants grew well, and the relative electrical conductivity of the transgenic plants was significantly lower compared to the control plants. The results showed that foreign genes have been successfully integrated into aloe genomic DNA and expressed stably.