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叔丁基脂红过氧化物（tBOOH）和百草枯（Paraquat）是一类具有细胞毒性作用的活性氧种，超氧化物歧化酶（MnSOD）和谷胱甘肽过氧化物酶（GPx）是细胞内清除活性氧种的重要抗氧化酶。本研究以稳定表达MnSOD与GPx的CHO细胞系为细胞模型，通过分析细胞存活能力和线粒体功能比较研究了MnSOD与GPx对tBOOH和Paraquat诱导细胞死亡的不同影响。结果表明，MnSOD能选择性地阻断Paraquat的细胞毒作用，而GPx则选择性地阻断tBOOH的细胞毒性作用。该结论揭示了不同的活性氧物质通过不同的机制诱发细胞死亡，而选择不同的抗氧化酶能特异地阻断活性氧的细胞毒性作用。同时提示，保护线粒体功能可能是抗氧化酶保护细胞免受活性氧种损伤的一个重要方面。
T-Butyl lipid peroxide (tBOOH) and paraquat are a group of cytotoxic reactive oxygen species, with superoxide dismutase (MnSOD) and glutathione peroxidase (GPx) being Intracellular clearance of reactive oxygen species important antioxidant enzymes. In this study, CHO cell lines stably expressing MnSOD and GPx were used as cell models. The effects of MnSOD and GPx on tBOOH and Paraquat-induced cell death were compared by analyzing the cell viability and mitochondrial function. The results show that MnSOD can selectively block Paraquat cytotoxicity, while GPx selectively block the cytotoxicity of tBOOH. This conclusion reveals that different ROS species induce cell death through different mechanisms, and that different antioxidant enzymes can specifically block the cytotoxic effect of reactive oxygen species. At the same time suggest that protection of mitochondrial function may be an important aspect of antioxidant enzymes to protect cells from reactive oxygen species damage.