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目的 对细梗香草(Lysimachia capillipes Hemsl.)全草的三萜皂苷成分进行分离和结构鉴定。方法 通过大孔树脂纯化,硅胶和反相硅胶色谱分离正丁醇萃取物中的三萜皂苷;利用多种波谱技术并结合酸水解方法鉴定其化学结构。结果 分离鉴定了2个三萜皂苷,其结构分别为3β,22α-二羟基-16α-当归酰氧基-28→13-内酯-齐墩果烷-3-O-[β-D-吡喃葡糖基-(1→2)-α-L-吡喃阿拉伯糖基]-22-O-(6-乙酰基)-β-D-吡喃葡糖苷(Ⅰ),3β,13β,22α-三羟基-16α-乙酰氧基-齐墩果烷-28羧酸钠-3-O-[β-D-吡喃葡糖基-(1→2)-α-L-吡喃阿拉伯糖基]-22-O-β-D-吡喃葡糖苷(Ⅱ)。结论 化合物Ⅰ和Ⅱ为新的三萜皂苷,分别命名为细梗香草皂苷Ⅰ和细梗香草皂苷J。
Objective To isolate and identify the triterpene saponins from Lysimachia capillipes Hemsl. Methods The triterpene saponins in n-butanol extract were separated by macroporous resin purification, silica gel and reversed-phase silica gel chromatography. Their chemical structures were identified by a variety of spectroscopic techniques combined with acid hydrolysis. Results Two triterpene saponins were isolated and identified. Their structures were 3β, 22α-dihydroxy-16α-angelica-oxyl-28→13-lactone- oleanan-3-O-[β-D-pyrazole, respectively. Glucopyranosyl-(1→2)-α-L-glucopyranosyl]-22-O-(6-acetyl)-β-D-glucopyranoside (I), 3β, 13β, 22α -Sodium trihydroxy-16α-acetoxy-oleozan-3-carboxylate-3-O-[β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl ]-22-O-β-D-glucopyranoside (II). Conclusion Compounds I and II are new triterpene saponins, and they are named as the fine stem vanilla saponin I and the fine stem vanilla saponin J respectively.