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目的 建立鼠疫耶尔森菌的蛋白质组学研究方法 ,获得鼠疫耶尔森菌的基本蛋白质组数据。方法 以对人无致病能力的布氏田鼠疫源地菌株 910 0 1为研究对象 ,按照溶解性的不同分别收集菌体蛋白 ,以 3种不同方法 (Shotgun LC MS MS ,1D LC MS MS和2D MS)进行分析 ,将分析数据与基于 910 0 1菌株基因组全序列建立的蛋白质理论数据库进行比对 ,确定 910 0 1菌株本实验培养条件下所表达的蛋白组分。结果 Shotgun LC MS MS方法鉴定了 971种蛋白 ,1D LC MS MS鉴定了 915种 ,而 2D MS方法则鉴定了 2 33种蛋白质 ,三者合计为 1193种蛋白质 ,占基因组预测CDS的 2 8 7% (1193/414 3)。结论 不同的蛋白质组分析方法鉴定的蛋白质种类和数目都存在差异 ,为更全面地获得鼠疫耶尔森菌蛋白质组数据 ,有必要同时采取多种方法进行分析。
Objective To establish a proteomics research method for Yersinia pestis and obtain the basic proteome data of Yersinia pestis. Methods The bacterial strain 910 0 1 of Brandt’s vole (Microtus brandti), which was not pathogenic to humans, was collected and the bacterial proteins were collected according to their solubilities. Three different methods (Shotgun LC MS MS, 1D LC MS MS and 2D MS). The data were compared with the theoretical database of proteins based on the complete genome sequence of strain 910 0 1 to determine the protein components of 910 0 1 strain cultured in this experiment. Results 971 proteins were identified by Shotgun LC MS MS, 915 were identified by 1D LC MS MS, 2 33 proteins were identified by 2D MS method, and the total of 1193 proteins accounted for 287% of the genome predicted CDS. (1193/414 3). Conclusion There are differences in the types and numbers of proteins identified by different proteome analysis methods. In order to obtain the Yersinia pestis proteome data more comprehensively, it is necessary to adopt a variety of methods simultaneously for analysis.