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目的诱导表达和纯化细粒棘球蚴Eg95抗原重组蛋白,对其与CE病人血清学反应情况进行研究。方法IPTG诱导pET28a-Eg95原核表达质粒,表达和纯化Eg95重组蛋白,SDS-PAGE电泳对其进行初步鉴定,Westernblot法检测其与中间宿主CE病人血清学反应情况。结果pET28a-Eg95重组蛋白得到成功表达,SDS-PAGE电泳显示相对分子量约为15kDa。Westernblot结果表明,pET28a-Eg95重组蛋白能被CE病人血清识别,11例CE病人血清中8例呈阳性反应。结论细粒棘球蚴Eg95抗原存在于中间宿主CE病人体内,Eg95蛋白作为保护性抗原可能成为CE病人术后辅助性治疗的手段之一。
Objective To induce the expression and purification of recombinant protein of Eg95 antigen of Echinococcus granulosus and study its serological response to CE patients. Methods The plasmid pET28a-Eg95 was induced by IPTG, and the recombinant protein Eg95 was expressed and purified. SDS-PAGE electrophoresis was used to identify the recombinant protein. Western blotting was used to detect the serological reaction of the recombinant protein with the intermediate host CE. Results The recombinant protein pET28a-Eg95 was successfully expressed. SDS-PAGE electrophoresis showed that the relative molecular weight was about 15kDa. The results of Western blot showed that the recombinant protein of pET28a-Eg95 could be recognized by the serum of CE patients, and the serum of 8 of 11 patients with CE was positive. Conclusion Eg95 antigen of Echinococcus granulosus is present in intermediate host CE patients, and Eg95 protein may be one of the adjuvant adjuvant treatments for CE patients as a protective antigen.