论文部分内容阅读
目的:研究高血压候选基因β3肾上腺素能受体基因β3-Adrenergic receptor(ADRB3)在日本人群的分布,并探讨ADRB3基因Trp64Arg多态性与原发性高血压病(essential hypertension,EH)的关系.方法:采用流行病学调查方法分析肥胖指数、血浆胆固醇和甘油三酯等临床指标,同时调查受检者吸烟和饮酒等生活习惯;Taqman-PCR法分析ADRB3基因Trp64Arg多态性在大样本日本人群(样本总数2 714人,其中HT组1 328例,非高血压组(NT)1 386例)的分布及其与临床指标间的相关性.结果:除总胆固醇外,其余指标如年龄、性别、肥胖指数、舒张期血压、收缩期血压、HDL-脂蛋白和甘油三酯等在Trp/Trp、Trp/Arg和Arg/Arg这3类基因型携带者中均无显著性差异;卡方检验结果显示ADRB3的Trp64Arg基因型(p=0.425)与等位基因频率(p=0.501)在HT与NT之间没有显著统计学意义;ADRB3的Trp64Arg基因多态性与高血压病Logistic回归模型分析结果显示,卡方值(Wald)为0.356;比值比为0.884;95%的可信区间为0.589~1.326,p值为0.551.结论:按照p<0.05统计学标准,ADRB3的Trp64Arg多态性可能与日本人群EH无关.
Objective: To investigate the distribution of β3-adrenergic receptor β3 adrenoceptor gene (ADRB3) in Japanese population and to explore the relationship between the ADRB3 Trp64Arg polymorphism and essential hypertension (EH) Methods: Epidemiological methods were used to analyze the clinical indexes such as obesity index, plasma cholesterol and triglyceride, and smoking and drinking habits were also investigated. The Taqman-PCR analysis of the Trp64Arg polymorphism of ADRB3 gene in large sample Japan The distribution of the population (with a total sample of 2 714 samples including 1 328 cases of HT group and 1 386 cases of non-hypertensive group) and its correlation with clinical parameters were analyzed.Results: Except for total cholesterol, other indicators such as age, There was no significant difference in gender, obesity index, diastolic blood pressure, systolic blood pressure, HDL-lipoprotein and triglyceride between the three genotypes Trp / Trp, Trp / Arg and Arg / Arg. The results showed that the Trp64Arg genotype (p = 0.425) and the allele frequency (p = 0.501) of ADRB3 had no significant difference between HT and NT. The polymorphism of Trp64Arg gene of ADRB3 and Logistic regression model of hypertension The results show, The Wald value was 0.356, the odds ratio was 0.884, the 95% confidence interval was 0.589 to 1.326, and the p value was 0.551.Conclusion: According to the p <0.05 statistical criteria, the Trp64Arg polymorphism of ADRB3 may be associated with the EH Nothing to do