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目的 探测白细胞介素 - 6 (IL- 6 )是否与垂体前叶中的神经纤维的营养效应有关。 方法 应用免疫组织化学和原位杂交技术对去肾上腺大鼠垂体中的 IL- 6表达进行形态观察 ,并试用两种微量 PCR方法对同一组织中的 IL- 6进行蛋白水平和 m RNA水平的定量检测。 结果 免疫组织化学分析 ,阳性物质主要存在于垂体前叶滤泡星形 (FS)细胞中 ,但其含量变化却不甚清晰。PCR定量分析 ,IL- 6在上述两个水平上的表达均有增加 ,且基因激活时间早于术后 2 4h。 结论 IL- 6变化与 ACTH无关 ,可能参与下丘脑 -垂体 -肾上腺 (HPA)轴以外的垂体前叶功能调节的其他路径 ,譬如通过神经支配而发挥影响
Objective To investigate whether interleukin - 6 (IL - 6) is involved in the nutritional effects of nerve fibers in the anterior pituitary. Methods Immunohistochemistry and in situ hybridization were used to observe the morphological changes of IL-6 in the pituitary of adrenal rats. Two kinds of micro-PCR methods were used to quantify the levels of IL-6 in the same tissues Detection. Results Immunohistochemical analysis showed that the positive substances mainly existed in the anterior pituitary follicle-like (FS) cells, but their content was not clear. PCR quantitative analysis, IL-6 in both expression levels were increased, and the gene activation time earlier than 24 h after surgery. CONCLUSION: The alteration of IL-6 is not related to ACTH and may be involved in other pathways regulating the function of the anterior pituitary outside the hypothalamus-pituitary-adrenal (HPA) axis, for example, through innervation