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目的 :研究大豆异黄酮对大鼠免疫细胞的体外作用。方法 :取大鼠抗凝血 ,密度梯度离心法分离外周血单个核细胞 ,以伴刀豆球蛋白A(ConA)和体内所能达到的不同浓度的金雀异黄素或大豆苷元共同孵育 6 8h后 ,四唑蓝还原法 (MTT)测定体外ConA诱导的淋巴细胞增殖 ;另取外周血单核细胞为效应细胞 ,YAC 1细胞为靶细胞 ,以乳酸脱氢酶法测定天然杀伤细胞 (NK )活性。结果 :在 0 .0 1～ 1μmol·L- 1浓度范围内 ,大豆苷元可剂量依赖性的增强体外ConA诱导的淋巴细胞增殖 (P <0 .0 1) ,1μmol·L- 1的大豆苷元可明显增强NK细胞活性 (P<0 .0 1)。而同样浓度范围内的金雀异黄素对淋巴细胞增殖及NK细胞活性均无明显作用。结论 :大豆苷元体外能剂量依赖性的刺激ConA诱导的淋巴细胞转化 ,增强NK细胞活性 ,这种增强的免疫功能可能是大豆制品抗肿瘤作用的机制之一
Objective: To study the in vitro effect of soy isoflavones on rat immune cells. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from rat blood by anticoagulant and density gradient centrifugation. Concanavalin A (ConA) was incubated with different concentration of genistein or daidzein in vivo Six hours later, MTT assay was used to detect the proliferation of lymphocytes induced by ConA in vitro. The peripheral blood mononuclear cells were taken as effector cells and YAC 1 cells as target cells. Lactate dehydrogenase method was used to detect natural killer cells NK) activity. RESULTS: Daidzein increased the ConA-induced lymphocyte proliferation (P <0.01) in vitro and daidzein (1 μmol·L -1) in a dose-dependent manner at a concentration of 0.1-1 μmol·L -1 Yuan significantly increased NK cell activity (P <0.01). Genistein at the same concentration range had no significant effect on lymphocyte proliferation and NK cell activity. Conclusion: Daidzein can stimulate ConA-induced lymphocyte transformation in vitro and enhance NK cell activity in a dose-dependent manner. This enhanced immune function may be one of the mechanisms of antitumor effect of soybean products