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目的:以1,25-二羟维生素D3诱导建立高钙血症动物模型。方法:将40只Wistar大鼠随机分为4组,每组10只。分别将1, 25-二羟维生素D3以高中低(4μg/kg,2μg/kg,0.5μg/kg)三个剂量组连续灌喂Wistar大鼠2周,空白对照组灌喂2 mL生理盐水眼球丛静脉取血后测定大鼠血钙值及血液的生理生化指标。结果:以2μg/kg浓度的1,25-二羟维生素D3灌喂之后,大鼠血钙值显著上升(P<0.05),同时其它生理生化指标和对照组无明显差异。注射降血钙药物1.25 mg/kg帕米膦酸二钠(pamidronate)或200mIU/kg密钙息(Miacalcic)均能有效抑制该模型大鼠的血钙升高。结论:采用1,25-二羟维生素D3为诱导物是建立实验性高钙血症大鼠模型的一种可行方法。
OBJECTIVE: To establish an animal model of hypercalcaemia induced by 1,25-dihydroxyvitamin D3. Methods: Forty Wistar rats were randomly divided into 4 groups with 10 rats in each group. The Wistar rats were respectively fed with three doses of 1, 25-dihydroxyvitamin D3 (4μg / kg, 2μg / kg, 0.5μg / kg) for 2 weeks. The blank control group was infused with 2ml saline eyeball Measurement of serum calcium and blood physiological and biochemical indexes after taking blood from cava. Results: After being fed with 1,25-dihydroxyvitamin D3 at a concentration of 2μg / kg, the serum calcium level increased significantly (P <0.05). At the same time, there was no significant difference between other physiological and biochemical indexes and the control group. Injection of hypocalcemic drugs 1.25 mg / kg pamidronate pamidronate (pamidronate) or 200mIU / kg Mi close (Miacalcic) can effectively inhibit the model of rat serum calcium. Conclusion: The use of 1,25-dihydroxyvitamin D3 as an inducer is a viable method to establish a rat model of experimental hypercalcemia.