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目的研究脉络膜黑色素瘤中PTEN蛋白表达及其与脉络膜黑色素瘤局部侵袭转移机制的相关性。设计回顾性病例系列。研究对象36例脉络膜黑色素瘤和2例正常葡萄膜组织。方法对36例脉络膜黑色素瘤进行组织病理学分析,应用免疫组化方法检测肿瘤组织及正常葡萄膜组织中PTEN的表达,并分析其与组织病理学特征以及同肿瘤侵犯巩膜导管与否之间的关系。主要指标病理组织学、免疫组织化学结果。结果36例脉络膜黑色素瘤组织中,肿瘤侵犯巩膜导管15例(41．7％), PTEN阳性表达20例(55．6％);2例正常葡萄膜组织全部阳性。巩膜导管受侵犯组和未受侵犯组阳性表达率分别为33．3％(5/15例)和76．2％(16／21例)(X2=5．143,P=0．026)。结论PTEN蛋白在脉络膜黑色素瘤侵犯巩膜导管时明显降低。推测PTEN基因的正常表达在抑制脉络膜黑色素瘤细胞的聚集黏附过程中具有重要作用。
Objective To investigate the expression of PTEN protein in choroidal melanoma and its relationship with the local invasion and metastasis of choroidal melanoma. Design retrospective case series. 36 cases of choroidal melanoma and 2 cases of normal uveal tissue. Methods 36 cases of choroidal melanoma were histopathologically analyzed. Immunohistochemistry was used to detect the expression of PTEN in tumor tissues and normal uveal tissues. The relationship between the expression of PTEN and histopathological features and the invasion of scleral duct with tumor relationship. The main indicators of histopathology, immunohistochemistry results. Results In 36 cases of choroidal melanoma, 15 cases (41.7%) had invasive lesions of the scleral duct and 20 cases (55.6%) of them had positive PTEN. 2 cases of normal uveal tissues were all positive. The positive rates of scleral duct invasion and noninvasive group were 33.3% (5/15 cases) and 76.2% (16/21 cases) respectively (X2 = 5.143, P = 0.026). Conclusions PTEN protein is significantly decreased when choroidal melanoma invades the scleral duct. It is speculated that the normal expression of PTEN gene plays an important role in inhibiting the aggregation and adhesion of choroidal melanoma cells.