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目的 探讨补肾通络方能否诱导去势大鼠阴茎组织细胞增殖增殖细胞包括成纤维细胞、平滑肌细胞及血管内皮细胞等。方法 40只成年SD雄性大白鼠随机分为对照组、去势组、补肾通络方组及丙酸睾酮组。补肾通络方组、丙酸睾酮组,术后2周分别灌胃补肾通络方药和肌注丙酸睾酮。各组分别于治疗后4 d和1周处死,取中断阴茎组织,用免疫组化技术(SP法)检测其细胞增殖情况。结果 对照组阴茎组织中存在中等强度的细胞增殖(35. 07±2. 94),去势2周后几乎无细胞增殖(P<0. 01);丙酸睾酮组、补肾通络方组治疗4 d和1周后即诱导DNA合成,细胞增殖显著增多(20 .60±2 66,19 .62±1 .75,P<0. 05)。结论 补肾通络方能诱导去势大鼠阴茎组织多种类型细胞增殖。
Objective To investigate whether Bushen Tongluo Fang can induce proliferation and proliferation of penile tissue in ovariectomized rats including fibroblasts, smooth muscle cells and vascular endothelial cells. Methods Forty male Sprague-Dawley rats were randomly divided into control group, castration group, Bushen Tongluo group and testosterone propionate group. Bushen Tongluo group and testosterone propionate group were given orally with Bushen Tongluo prescription and intramuscular injection of testosterone propionate 2 weeks after operation. Each group was sacrificed 4 days and 1 week after treatment, and the penis tissue was discontinued. The proliferation of the cells was detected by immunohistochemical technique (SP method). Results There was moderate-intensity cell proliferation in the penis of the control group (35.07±2.94). There was almost no cell proliferation after 2 weeks of ovariectomy (P<0.01); treatment with testosterone propionate and Bushentongluo Recipe After 4 days and 1 week, DNA synthesis was induced and cell proliferation was significantly increased (20.60±2.66, 19.62±1.75, P<0.05). Conclusion Bushentongluo can induce proliferation of many types of cells in the penis tissue of ovariectomized rats.