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目的:研究E-CD(E-cadherin,E钙黏着蛋白)、integrinα5β1(整联蛋白α5β1)、基质金属蛋白酶-2(m atrix m etalloprote inase-2,MMP-2)的表达联合DNA倍体和增殖指数(proliferation index,PI)的检测在肺癌侵袭和转移中的意义。方法:免疫组化法观察57例肺癌组织三指标的表达情况,同时用流式细胞术分析这57例组织的DNA倍体和PI。结果:E-CD在肺癌不同组织分化之间存在显著性差异(P<0.05)。E-CD、integrinα5β1、MMP-2在淋巴结转移情况都存在显著性差异(P<0.05)。流式细胞术结果显示PI在TNM分期、组织分化、组织类型中均存在显著性差异(P<0.05),但和淋巴结转移无关。DNA异倍体与淋巴结有无转移之间存在显著性差异(P<0.05)。结论:随着肿瘤异质性的发生和发展,E-CD、integrinα5β1、MMP-2的异常表达以及DNA异倍体率的增加,表明他们在肺癌侵袭和转移的发生中扮演着重要的角色。但本文研究显示PI的增加和淋巴结转移无关。
Objective: To investigate the expression of E-cadherin, integrinα5β1 (integrin α5β1), matrix metalloproteinase-2 (MMP-2) and DNA ploidy Significance of proliferation index (PI) detection in lung cancer invasion and metastasis. Methods: Immunohistochemistry was used to detect the expression of three markers in 57 cases of lung cancer. Meanwhile, the DNA ploidy and PI of these 57 cases were analyzed by flow cytometry. Results: There was a significant difference between E-CD in different tissues of lung cancer (P <0.05). E-CD, integrinα5β1, MMP-2 in lymph node metastasis were significantly different (P <0.05). Flow cytometry showed that there was a significant difference (P <0.05) between PI and TNM staging, tissue differentiation and histological type, but not with lymph node metastasis. There was a significant difference between DNA aneuploidy and lymph node metastasis (P <0.05). Conclusion: The abnormal expression of E-CD, integrinα5β1 and MMP-2 and the increase of DNA aneuploidy with the occurrence and development of tumor heterogeneity indicate that they play an important role in the invasion and metastasis of lung cancer. However, this study shows that the increase of PI has nothing to do with lymph node metastasis.