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双波长分光测定法是一种正在发展应用的光电比色法。它的主要特点是,采用两种不同波长的单色光,通过同一个样品杯,测定样品对该两波长的吸收差值。在测定光谱中总有干扰存在,诸如样品的额外吸收和散射,以及样品杯本身的非均匀性本底等产生的非特征吸收。双波长分光测定法可以将这些非特征吸收部分——背景干扰扣除,而保存特征吸收部分,从而提高了测量的灵敏度和精度。本文介绍用双波长分光测定法扣除由于比色杯散射本底和光学非均一性引起的测量误差的实验技术和方法。
Dual wavelength spectrophotometry is a growing application of photoelectric colorimetry. Its main feature is the use of two different wavelengths of monochromatic light, through the same sample cup, the determination of sample absorption difference between the two wavelengths. There is always interference in the measurement spectrum, such as non-specific absorption of the sample due to extra absorption and scattering, as well as the non-uniformity background of the sample cup itself. Dual-wavelength spectrophotometry can subtract these non-characteristic absorption-background interferences while preserving the characteristic absorption, thereby improving the sensitivity and accuracy of the measurement. This article describes experimental techniques and methods for using two-wavelength spectrophotometry to subtract measurement errors caused by the cuvette’s background and optical inhomogeneity.